In chromatography, no actual plates are used, although the notion of the theoretical plate, as a distinct region where an individual equilibrium is maintained, stays. In a specific liquid chromatographic separation, the number of theoretical plates and the peak equivalent to a theoretical plate (HETP) are similar merely by the size of the columnThe

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Mikhail Semyonovich Tsvet gets credit history for inventing liquid column chromatography. In 1901, he introduced an adsorption chromatography system for separating plant pigments with petroleum ether in the narrow glass tube crammed with calcium carbonate.Know your column: Columns have diverse pore sizes and solvent tolerances. Verify Together with

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